More data
Sequence all genomic variants including epigenetic modifications
Better results
Directly sequence long sections of DNA (>50 kbp) with high accuracy
Less complexity
Minimal Sample Prep and Reduced Reagent Consumption
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Compared to the ionic sensing of other nanopore methods, SERS-based detection provides direct structural information, enabling distinction of individual nucleotides and epigenetic, isotopic and other chemical modification markers
2
Enhanced Raman Spectroscopy (SERS) facilitates the direct measurement of the actual physical properties of in-situ nucleotides in nucleic acids.
3
Takes a complete spectral “Fingerprint” of each nucleotide and detects critical DNA modifications with distinct spectra - Epigenetic Modifications Directly Measured - example
dCTP, 5m-dCT, 5hm-dCTP.
Why SERS Raman?
Direct Long-Read Epigenetic Sequencing Platform
Addresses the limitations of today’s sequencing technologies to advance the promise of precision medicine.
Epigenetics, a rapidly expanding field of biology, involves the study of changes in gene expression that don't stem from changes in the DNA sequence, but rather from chemical modifications of DNA and its associated proteins.
01
Changes to the epigenome — DNA methylation, histone modifications, and the chromatin state — influence what genes are expressed
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Many changes, or modifications to one’s genome are not able to be read today. These modifications turn genes "on" or "off and play a vital role in a wide range of diseases, including cardiovascular disease, cancer, neurodevelopmental disorders, and neurodegenerative disorders.